NAD+: CELLULAR ENERGY AND ANTI-AGING

OVERVIEW:

• NAD+ (Nicotinamide Adenine Dinucleotide) is a coenzyme found in all living cells 

• Essential for energy production, DNA repair, and cellular metabolism 

• Naturally declines with age, decreasing approximately 50% by age 40-60

  
  

WHAT DOES NAD+ DO?

• Enhances cellular energy production and ATP synthesis 

• Supports DNA repair and genomic stability 

• Activates sirtuins for longevity and anti-aging effects 

• Improves mitochondrial function and biogenesis 

• Enhances cognitive function and mental clarity 

• Supports metabolic health and insulin sensitivity

  
  

USES:

• Anti-aging and longevity optimization 

• Energy enhancement and fatigue reduction 

• Cognitive function and mental clarity 

• Athletic performance and recovery 

• Metabolic health and weight management 

• Neurological support and neuroprotection 

• Addiction recovery support

  
  

NAD+ RESEARCH

Cellular Energy Production: NAD+ is crucial for cellular respiration and ATP production in mitochondria. It acts as an electron carrier in metabolic processes, converting nutrients into usable cellular energy through the electron transport chain.

DNA Repair and Genomic Stability: NAD+ is required for PARP enzyme function, which repairs DNA damage and maintains genomic integrity. Adequate NAD+ levels support cellular repair mechanisms and reduce age-related DNA damage accumulation.

Sirtuin Activation: NAD+ activates sirtuins (SIRT1-7), proteins that regulate cellular health, longevity, inflammation, and stress resistance. Sirtuin activation is linked to increased lifespan and healthspan in multiple organisms.

Age-Related Decline: NAD+ levels decline significantly with aging due to increased consumption by PARP and CD38 enzymes, reduced biosynthesis, and mitochondrial dysfunction. This decline contributes to age-related diseases and cellular dysfunction.

Neuroprotection: NAD+ supports brain health through enhanced mitochondrial function, reduced neuroinflammation, and improved synaptic plasticity. Studies suggest benefits for neurodegenerative conditions and cognitive decline.

Metabolic Benefits: NAD+ improves insulin sensitivity, enhances fat oxidation, and supports healthy glucose metabolism. It plays a crucial role in maintaining metabolic homeostasis and preventing metabolic syndrome.

Potential Mechanism of Action: NAD+ works through multiple pathways:

1. Serves as coenzyme for energy metabolism (glycolysis, TCA cycle, electron transport)

2. Activates sirtuin proteins for longevity and cellular health

3. Supports PARP enzymes for DNA repair

4. Enhances mitochondrial function and biogenesis

5. Regulates circadian rhythm and cellular stress responses

6. Modulates inflammation and immune function

NAD+ LIFESTYLE SUPPORT

LIFESTYLE SUPPORT: 

• Regular exercise to boost natural NAD+ production

• Adequate sleep (7-9 hours) for circadian rhythm and NAD+ synthesis

• Intermittent fasting or caloric restriction enhances NAD+ levels

• Limit excessive alcohol consumption (depletes NAD+)

• Reduce oxidative stress through antioxidant-rich diet

• Sun exposure for vitamin D and circadian support

• Stress management for reduced NAD+ consumption

WHAT TO EXPECT:

IMPORTANT: NAD+ effects build over time with consistent supplementation!

• Enhanced energy and reduced fatigue within days to weeks

• Improved mental clarity and cognitive function

• Better exercise performance and recovery

• Enhanced mood and stress resilience

• Long-term anti-aging and cellular health benefits

• Individual responses vary based on baseline NAD+ status

• Effects most noticeable in those with deficiency

NAD+ DOSING + CYCLING

Injectable NAD+ Protocol:

NAD+	Bacteriostatic water added	Strength mg/mL	Dose	Frequency	Supply
500 mg Vial	5 mL	100mg/mL	50-100mg	2-3x weekly	5-10 injections

Cycling: Continuous use typical, or 8-12 weeks on, 2-4 weeks off

• Subcutaneous or intramuscular injection

• Slow injection to minimize discomfort

• Start with lower doses to assess tolerance

• Can increase to 100-250mg for intensive protocols

NAD+ DOSING + CYCLING

IV Infusion Protocol (Clinical Setting):

NAD+	Saline/Fluid	Dose Range	Frequency	Duration
NAD+	250-500mL	250-1000mg	1-2x weekly	2-4 hours per infusion

Cycling: Weekly or bi-weekly infusions, ongoing or periodic intensive protocols

• Must be administered slowly (over 2-4 hours)

• Clinical supervision required

• Higher bioavailability than other methods

• More expensive but most effective delivery

NAD+ PRECURSOR ALTERNATIVES:

Oral NAD+ Precursors (Non-Injectable):

Compound	Typical Dose	Frequency	Bioavailability
NMN	250-500mg	Daily	Moderate-High
NR	300-500mg	Daily	High
Niacin	50-500mg	Daily	Moderate (flushing)
Niacinamide	500-1000mg	Daily	Moderate

Note: Oral precursors convert to NAD+ in cells and may be more practical for long-term use

NAD+: SCREENING

NAD+ is generally safe but requires consideration of specific conditions.

Screening Questions: 

Do you have any cardiovascular conditions or arrhythmias?

Are you pregnant or breastfeeding?

Do you have a history of cancer (especially hormone-sensitive)?

Are you taking blood pressure or diabetes medications?

Reasons: 

NAD+ can affect cardiovascular function and blood pressure

Safety during pregnancy/breastfeeding not established

Sirtuin activation effects on cancer cells not fully understood

May affect glucose metabolism and blood pressure regulation

Note: NAD+ infusions can cause temporary side effects including nausea, flushing, cramping, or anxiety during administration. Starting with lower doses and slower infusion rates minimizes these effects. Injectable NAD+ is generally better tolerated than IV infusions.